I-Aspergillus, iCryptococcus Neoformans, iCandida Albicans Uvavanyo lweMolekyuli (i-PCR yexesha lokwenyani) Umfanekiso obonakalayo

Aspergillus, Cryptococcus Neoformans, Candida Albicans Uvavanyo lweMolekyuli (Ixesha lokwenyani PCR)

Uvavanyo lwePCR oluchanekileyo lweMucorales.

Izinto zokubhaqa	Mucorales spp.
Indlela yokusebenza	I-PCR yexesha langempela
Uhlobo lwesampuli	Isikhohlela, ulwelo lwe-BAL, iSerum
Iinkcukacha	Iimvavanyo ezingama-20/ikhithi, iimvavanyo ezingama-50/ikhithi
Ikhowudi yemveliso	I-FMPCR-20, i-FMPCR-50

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Intshayelelo yeMveliso

I-Aspergillus, i-Cryptococcus Neoformans, i-Candida Albicans Uvavanyo lweMolekyuli (i-PCR yexesha langempela) iyasebenza ekubhaqweni kobungakanani be-DNA ye-Aspergillus, i-Cryptococcus neoformans kunye ne-Candida albicans kwi-bronchoalveolar lavage.Ingasetyenziselwa uxilongo oluncedisayo lwe-Aspergillus, i-Cryptococcus neoformans kunye ne-Candida albicans kunye nokubeka iliso kwisiphumo sonyango lonyango lwezigulana ezosulelekileyo.

Iimpawu

Igama	Aspergillus, Cryptococcus Neoformans, Candida Albicans Uvavanyo lweMolekyuli (Ixesha lokwenyani PCR)
Indlela	I-PCR yexesha langempela
Uhlobo lwesampuli	BAL ulwelo
Inkcazo	Iimvavanyo ezingama-50/ikhithi
Ixesha lokubona	2 h
Izinto zokubhaqa	Aspergillus, Cryptococcus Neoformans, Candida Albicans
Uzinzo	Izinzile kangangeenyanga ezili-12 kwi -20°C

Aspergillus, Cryptococcus Neoformans, Candida Albicans Uvavanyo lweMolekyuli (Ixesha lokwenyani PCR)

Inzuzo

Iluncedo
Isampulu yonyango lwangaphambili yenza lula ukutsalwa kwe-nucleic acid
Imisebenzi emininzi
Khangela iAspergillus, Cryptococcus Neoformans kunye neCandida Albicans ngaxeshanye

Ichanekile
1. I-reagent igcinwe kwi-tube ye-PCR ukunciphisa amathuba okungcola
2. Ulawula ngokungqongqo umgangatho wokulinga ngolawulo oluthathu lomgangatho.

Malunga nesifo sokungunda esihlaselayo

Ifungi liqela eliguquguqukayo leemicroorganisms ezinokubakho ngokukhululekileyo kokusingqongileyo, zibe yinxalenye yezityalo eziqhelekileyo zomntu nezilwanyana kwaye zikwazi ukubangela usulelo olungeyongozi olungeyongozi kusulelo oluyingozi oluyingozi.Usulelo lomngundo oluhlaselayo (IFI's) lolo usulelo apho umngundo uye wahlasela kwizicubu ezinzulu kwaye wazimisela ngokwawo okhokelela kwisigulo eside.Ii-IFI's zidla ngokubonwa kubantu abakhubazekileyo kunye nabangamajoni omzimba.Zininzi iingxelo ze-IFI's nkqu nakubantu abangenamandla okuzikhusela ngoko ke benza ukuba i-IFI ibe sisoyikiso esinokubakho kule nkulungwane yangoku.

Rhoqo ngonyaka, uCandida, Aspergillus kunye neCryptococcus bosulela izigidi zabantu kwihlabathi liphela.Uninzi lwamajoni omzimba abuthathaka okanye bagula kakhulu.I-Candida yeyona pathogen ixhaphakileyo yokungunda kwabo bagula kakhulu kwaye bafumana amalungu esisu afakelweyo.I-aspergillosis ehlaselayo ihlala isesona sifo sigqugqisayo sokungunda (i-IFD) sezigulane ze-haemato-oncological kunye nabamkeli be-organ-organ transplant kwaye ifumaneka ngokunyuka kubantu abanesifo semiphunga esingapheliyo kwi-corticosteroids.I-Cryptococcosis isesisifo esixhaphakileyo nesiyingozi kakhulu kubantu abane-HIV.

Uninzi losulelo lomngundo luye lwenzeka ngengozi kwaye usulelo lwenkqubo lomngundo lunqabile olunokuthi lube neziphumo zokusweleka okuphezulu.Kwinkqubo yosulelo lomngundo isiphumo sesi sifo sixhomekeke ngakumbi kwimiba yomkhosi kunokuba intsholongwane yomngundo.Ukusabela kwamajoni omzimba kusulelo lomngundo sisifundo esintsonkothileyo apho ukuhlasela komngundo kungaqondwanga yinkqubo yomzimba yokuzikhusela kwaye usulelo lomngundo oluhlaselayo lunokukhokelela kwiziphumo ezibi kakhulu ezikhokelela ekuguleni nasekufeni.Ukususela ekubeni iqhelekile ekuqaleni kwenkulungwane yama-20 xa ihlabathi lalithwaxwe ngubhubhani weentsholongwane, ukungunda kuye kwavela njengengxaki enkulu yezempilo yehlabathi.