Olu luhlu lweendlela luvavanyo ngokusebenzisa i-antigen ethile yentsholongwane ukufumanisa izilwa-buhlungu kwiserum yezigulana, kubandakanywa ubhaqo lwe-IgM antibodies kunye ne-IgG antibodies measurement.Ii-antibodies ze-IgM ziyanyamalala kwiiveki ezininzi, ngelixa izilwa-buhlungu ze-IgG ziqhubeka iminyaka emininzi.Ukuseka uxilongo losulelo lwentsholongwane kufezekiswa ngokwe-serologically ngokubonisa ukunyuka kwe-antibody titer kwintsholongwane okanye ngokubonisa izilwa-buhlungu ze-antiviral zodidi lwe-IgM.Iindlela ezisetyenzisiweyo ziquka uvavanyo lwe-neutralization (Nt), uvavanyo lwe-complement fixation (CF), uvavanyo lwe-hemagglutination inhibition (HI), kunye novavanyo lwe-immunofluorescence (IF), i-hemagglutination ye-passive, kunye ne-immunodiffusion.
A. Iimvavanyo zokungathathi hlangothi
Ngexesha losulelo okanye inkcubeko yeseli, intsholongwane inokuthintelwa yi-antibody ethile kwaye ilahlekelwe kukosuleleka, olu hlobo lwe-antibody luchazwa njenge-antibody neutralization.Uvavanyo lwe-neutralization kukubona i-antibody ye-neutralization kwi-serum yezigulana.
B. Ukuzalisekisa iiMvavanyo zoLungiso
Uvavanyo lokulungisa oluhambelanayo lunokusetyenziselwa ukujonga ubukho be-antibody ethile okanye i-antigen kwiserum yesigulana.Uvavanyo lisebenzisa iiseli zegazi ezibomvu zegusha (SRBC), isilwa-mzimba esichasene ne-SRBC kunye nephelelisi, kunye ne-antigen ethile (ukuba ijonge isilwa-mntu kwiserum) okanye isilwa-mntu esikhethekileyo (ukuba ujonge i-antigen kwiserum).
C. Ii-Hemagglutination Inhibition Assays
Ukuba uxinaniso lwentsholongwane kwisampulu luphezulu, xa isampulu ixutywa neeRBCs, iletisi yeentsholongwane kunye neeRBCs ziyakwenziwa.Le nto ibizwa ngokuba yi-hemagglutination.Ukuba ii-antibodies ezichasene ne-hemagglutinins zikhona, i-hemagglutination iya kuthintelwa.Ngexesha lovavanyo lwe-hemagglutination inhibition, i-serial dilutions ye-serum ixutywe kunye nenani elaziwayo lentsholongwane.Emva kokufukanyiswa, ii-RBCs zongezwa, kwaye umxube ushiywe ukuba uhlale iiyure ezininzi.Ukuba i-hemagglutination inqandekile, i-pellet ye-RBCs yenza iifom ezantsi kombhobho.Ukuba i-hemagglutination ayinqandwa, ifilimu encinci yenziwe.
Ixesha lokuposa: Oct-15-2020