Ukusukela ngoJanuwari 2020 ukuya kuOkthobha wama-2020, uphononongo olulindelekileyo lwendlela yokusebenza lwaqhutywa kwiSibhedlele seYunivesithi yasePisa olwapapashwa kwi-BMC Microbiology.Goldstream®I-Fungus (1-3) -β-D-Glucan Test yayisetyenziselwa ukufumanisa inqanaba le-BDG kwiisampuli ze-BAL.Iziphumo zalinganiswa ngu-aI-Automatic Kinetic Tube Reader IGL-200ukusuka kwi-Era Biology.Uphando lubonisa ukuba i-BDG kufuneka ithathelwe ingqalelo ngenxa yexabiso layo eliphezulu elibi.Kwaye kube luncedo ekulawuleni ukuxilongwa kwe-PCP kuzo zonke izigulane zolawulo olubi.
Imvelaphi:
Umgangatho okhoyo woxilongo wegolide wePneumocystis jiroveciiimelwe kukubonwa kwe-microscopic yefungus kwiisampulu zokuphefumla zeklinikhi, njenge-bronchoalveolar-lavage fluid, echaza "iqinisekisiwe"P. jiroveciiinyumoniya, ngelixa i-qPCR ivumela ukucacisa uxilongo “olunokwenzeka”, njengoko ingakwazi ukucalula usulelo olusuka kukholoni.Nangona kunjalo, iindlela zemolekyuli, ezinjengendawo yokuphela kwe-PCR kunye ne-qPCR, ziyakhawuleza, zisebenza ngokulula kwaye zitolika, ngaloo ndlela zivumela ilabhoratri ukuba ibuyisele ugqirha idatha eluncedo ye-microbiological ngexesha elifutshane.Uphononongo lwangoku lujolise ekuthelekiseni i-microscopy kunye novavanyo lwemolekyuli kunye ne-beta-D-glucan yokuxilonga ukusebenza kwi-bronchoalveolar-lavage fluids evela kwizigulana ezikrokrelekayo.Pneumocystis jiroveciiukukrala kwemiphunga.I-Bronchoalveolar-lavage fluid ukusuka kwishumi elinesibhozo eliphezulu kunye nezifundo ezine ezingalunganga zolawulo ziye zafumana i-methenamine yesilivere yaseGrocott-Gomori, i-PCR yokuphela, i-RT-PCR, kunye ne-beta-D-glucan assay.
Iziphumo:
Zonke iisampulu ze-microscopically positive bronchoalveolar-lavage (50%) nazo zibe neziphumo ezilungileyo nge-PCR yexesha lokuphela kunye nexesha lokwenyani kunye nazo zonke, kodwa ezimbini, zibe neziphumo ezilungileyo kwakhona nge-beta-D-glucan quantification.I-PCR yokuphela kunye ne-RT-PCR ifumene i-10 (55%) kunye ne-11 (61%) kwiisampuli ze-18, ngokulandelanayo, ngaloo ndlela ibonisa uvakalelo oluphuculweyo xa kuthelekiswa ne-microscopy.Yonke i-RT-PCR ene-Ct<27 yaqinisekiswa nge-microscopically, kanti iisampuli ezine-Ct≥ 27 zazingekho.
Izigqibo:
Umsebenzi wethu uqaqambisa imfuno yohlengahlengiso kunye nokuchaza kwakhona indima yoxilongo lwemolekyuli kwimeko ekhethekileyo yeklinikhi, njengaye.P. jiroveciiusulelo, olunqabileyo kodwa olukwanzima kwaye imeko yeklinikhi eqhubela phambili ngokukhawuleza echaphazela iinginginya ezingakwaziyo ukugoba ezinokuthi zixhamle kuxilongo olukhawulezayo.Izigulane ezikhethiweyo ngokungqongqo, ngokwemiqathango yokubandakanywa, umphumo ongalunganga ngeendlela ze-molecular unokukhutshwa ngaphandleP. jiroveciiukukrala kwemiphunga.
Franconi I, Leonildi A, Erra G, et al.Ukuthelekiswa kweenkqubo ezahlukeneyo ze-microbiological zokuxilongwa kwe-Pneumocystis jirovecii pneumonia kwi-bronchoalveolar-lavage fluid.I-BMC Microbiol.2022;22(1):143.Ipapashwe 2022 May 21. doi:10.1186/s12866-022-02559-1
Ixesha lokuposa: Jun-14-2022